Acute Phase Proteins in the Acute Phase Response - download pdf or read online

By J. Gauldie (auth.), M. B. Pepys MA, MD, PhD, FRCP, MCRPath (eds.)

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HepG2 and Hep3B) do not express SAA specific mRNA detectable by Northern analysis. The increase in specific mRNA is at least 50-fold by Northern analysis of total RNA extracted from stimulated transfectants. These results are similar to those described for murine hepatocyte cultures by Ramadori et al. (1985). In view of the positive results obtained above, we decided to use this in vitro model to observe the effect of other cytokines on the expression of this particular human SAA gene. 1 shows that y-interferon( y-IFN) , recombinant interleukin-6 (IL-6), and the phorbol ester, phorbol 12-myristate 13-acetate (PMA), all produce an increase in specific SAA mRNA.

1987) Interleukin-l modulates RNA levels oflymphokines and of other molecules associated with T cell activation in the T cell lymphoma LBRM 33-IA5. J Immunol 138:2514-2519 Hansen DF, Murphy PA, Silicano R, Shin HS (1983) The effect of temperature on the activation of thymocytes by interleukin 1 and 2. J Immunol 130:216-221 Helle M, Brakenhoff JPJ, DeGroot ER, Aarden LA (1988) Interleukin 6 is involved in interleukin 1induced activities. Eur J Immunol 18:957-959 Herrmann F, Oster W, Meuer SC, Klein K, Lindemann A, Mertelsmann R (1988) IL-l stimulates Tlymphocytes to produce GM-CSF.

Thus CRP opsonizes bacteria, parasites and plasma membranes, thereby facilitating their clearance by phagocytic cells. It has been also postulated that these molecules play an important role in the complexation, followed by phagocytosis, to DNA (Robey et al. 1984) released from lysed cells at the site of inflammation. In addition, some of the major acute phase reactants are active as general modulators of the immune response (James 1980; Hudig et al. 1981; Ades et al. Systems for the Study of the Acute Phase Response 31 1982; Pepys and Baltz 1983; Robey and Jones 1986).

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